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3d gelma biomimetic tooth bud constructs  (BioMimetic Therapeutics)

 
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    BioMimetic Therapeutics 3d gelma biomimetic tooth bud constructs
    A. DE and DM cells were seeded on thermo-responsive plates and cultured in normal DE and DM media, respectively, for 14 days. DE and DM CSs were detached by temperature reduction (20ºC) and layered over <t>GelMA</t> constructs to create experimental <t>3D</t> tooth bud constructs (CSG = DE and DM CSs layered over dental cells encapsulated in GelMA; G = GelMA alone). For in vivo analyses, replicate constructs were cultured in osteogenic media for 4 days and implanted subcutaneously onto the backs of the rats. B. Bioengineered 3D CS - GelMA tooth bud model. The bottom layer mimics the pulp organ (5% GelMA encapsulating DM cells) and the top layer mimics the enamel organ (3% GelMA encapsulating DE cells). The DE and DM CS layers mimic polarized DE-DM cell layers normally observed in developing teeth. C. Steps used to prepare the constructs. DM cells (3×107 cells/ml) were re-suspended in 100 μL of 5% GelMA and photo-crosslinked. DM and DE cell sheets were layered over the polymerized DM 5% GelMA. DE cells (3×107 cells/ml) re-suspended in 100 μL 3% GelMA and 100 μL, layered over construct and photo-crosslinked.
    3d Gelma Biomimetic Tooth Bud Constructs, supplied by BioMimetic Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3d gelma biomimetic tooth bud constructs/product/BioMimetic Therapeutics
    Average 90 stars, based on 1 article reviews
    3d gelma biomimetic tooth bud constructs - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Dental Cell Sheet Biomimetic Tooth Bud Model"

    Article Title: Dental Cell Sheet Biomimetic Tooth Bud Model

    Journal: Biomaterials

    doi: 10.1016/j.biomaterials.2016.08.024

    A. DE and DM cells were seeded on thermo-responsive plates and cultured in normal DE and DM media, respectively, for 14 days. DE and DM CSs were detached by temperature reduction (20ºC) and layered over GelMA constructs to create experimental 3D tooth bud constructs (CSG = DE and DM CSs layered over dental cells encapsulated in GelMA; G = GelMA alone). For in vivo analyses, replicate constructs were cultured in osteogenic media for 4 days and implanted subcutaneously onto the backs of the rats. B. Bioengineered 3D CS - GelMA tooth bud model. The bottom layer mimics the pulp organ (5% GelMA encapsulating DM cells) and the top layer mimics the enamel organ (3% GelMA encapsulating DE cells). The DE and DM CS layers mimic polarized DE-DM cell layers normally observed in developing teeth. C. Steps used to prepare the constructs. DM cells (3×107 cells/ml) were re-suspended in 100 μL of 5% GelMA and photo-crosslinked. DM and DE cell sheets were layered over the polymerized DM 5% GelMA. DE cells (3×107 cells/ml) re-suspended in 100 μL 3% GelMA and 100 μL, layered over construct and photo-crosslinked.
    Figure Legend Snippet: A. DE and DM cells were seeded on thermo-responsive plates and cultured in normal DE and DM media, respectively, for 14 days. DE and DM CSs were detached by temperature reduction (20ºC) and layered over GelMA constructs to create experimental 3D tooth bud constructs (CSG = DE and DM CSs layered over dental cells encapsulated in GelMA; G = GelMA alone). For in vivo analyses, replicate constructs were cultured in osteogenic media for 4 days and implanted subcutaneously onto the backs of the rats. B. Bioengineered 3D CS - GelMA tooth bud model. The bottom layer mimics the pulp organ (5% GelMA encapsulating DM cells) and the top layer mimics the enamel organ (3% GelMA encapsulating DE cells). The DE and DM CS layers mimic polarized DE-DM cell layers normally observed in developing teeth. C. Steps used to prepare the constructs. DM cells (3×107 cells/ml) were re-suspended in 100 μL of 5% GelMA and photo-crosslinked. DM and DE cell sheets were layered over the polymerized DM 5% GelMA. DE cells (3×107 cells/ml) re-suspended in 100 μL 3% GelMA and 100 μL, layered over construct and photo-crosslinked.

    Techniques Used: Cell Culture, Construct, In Vivo

    H&E images (A, B, C, D) revealed extracellular matrix formation and the morphology of the DE and DM cell sheets within the bilayer GelMA constructs. The arrows indicate the DE and the DM CSs. Pol images (E, F, G, H) show the organized collagen in the extracellular matrix. IF imaging (I, J, K, L) showed the expression of VM (green) by DM cells and CK18 (red) by the DE cells.
    Figure Legend Snippet: H&E images (A, B, C, D) revealed extracellular matrix formation and the morphology of the DE and DM cell sheets within the bilayer GelMA constructs. The arrows indicate the DE and the DM CSs. Pol images (E, F, G, H) show the organized collagen in the extracellular matrix. IF imaging (I, J, K, L) showed the expression of VM (green) by DM cells and CK18 (red) by the DE cells.

    Techniques Used: Construct, Imaging, Expressing

    High magnification H&E images and IHC analyses of multilayered DE DM CSs GelMA constructs cultured in osteogenic media for 24 h and 4 days, stained with FAK, TEN and SYN4. Arrows indicate expression. Cell sheets are identified as epithelial (DE) and mesenchymal (DM). A. H&E stained DE DM CSs GelMA constructs cultured in osteogenic media for 24 h. FAK, TEN and SYN4 staining (B, C and D) were detected in the DM CSs cultured in osteogenic media for 24 h. E. H&E image of DE DM CSs GelMA constructs cultured in osteogenic media for 4 days. F. FAK staining was detected in DE and DM CSs cultured in osteogenic media for 4 days. G. TEN was detected in the DM CSs cultured in osteogenic media for 4 days. H. Faint SYN4 staining was detected in DM CSs cultured in osteogenic media for 4 days. I. No staining was detected in the negative controls. Specific staining was detected on the natural tooth bud (J. FAK, K. TEN and L. SYN4).
    Figure Legend Snippet: High magnification H&E images and IHC analyses of multilayered DE DM CSs GelMA constructs cultured in osteogenic media for 24 h and 4 days, stained with FAK, TEN and SYN4. Arrows indicate expression. Cell sheets are identified as epithelial (DE) and mesenchymal (DM). A. H&E stained DE DM CSs GelMA constructs cultured in osteogenic media for 24 h. FAK, TEN and SYN4 staining (B, C and D) were detected in the DM CSs cultured in osteogenic media for 24 h. E. H&E image of DE DM CSs GelMA constructs cultured in osteogenic media for 4 days. F. FAK staining was detected in DE and DM CSs cultured in osteogenic media for 4 days. G. TEN was detected in the DM CSs cultured in osteogenic media for 4 days. H. Faint SYN4 staining was detected in DM CSs cultured in osteogenic media for 4 days. I. No staining was detected in the negative controls. Specific staining was detected on the natural tooth bud (J. FAK, K. TEN and L. SYN4).

    Techniques Used: Construct, Cell Culture, Staining, Expressing

    H&E images and IF analyses of multilayered DE DM CSs GelMA constructs cultured in osteogenic media for 24 h and 4 days, stained with SHH, RUNX2 and BMP2 in green, and VM positive DM cells in red. The red staining identifies the DM CSs, while, the absence of red staining identifies the DE cells. Arrows indicate expression. H&E stained DE DM CSs GelMA constructs cultured in osteogenic media for 24 h (A) and 4 days (E). SHH staining was detected in DE and DM CSs after 24 h (B) and 4 days (F). RUNX2 staining was faintly detected at the interface of DE and DM CSs (C), but strongly detected at the second layer of DE CSs after 24 h (inset in the image C), and detected in DE and DM CSs after 4 days (G). BMP2 staining was detected in DE and DM CSs after 24 h (D) and 4 days (H). No staining was detected in the negative controls (I, J and K).
    Figure Legend Snippet: H&E images and IF analyses of multilayered DE DM CSs GelMA constructs cultured in osteogenic media for 24 h and 4 days, stained with SHH, RUNX2 and BMP2 in green, and VM positive DM cells in red. The red staining identifies the DM CSs, while, the absence of red staining identifies the DE cells. Arrows indicate expression. H&E stained DE DM CSs GelMA constructs cultured in osteogenic media for 24 h (A) and 4 days (E). SHH staining was detected in DE and DM CSs after 24 h (B) and 4 days (F). RUNX2 staining was faintly detected at the interface of DE and DM CSs (C), but strongly detected at the second layer of DE CSs after 24 h (inset in the image C), and detected in DE and DM CSs after 4 days (G). BMP2 staining was detected in DE and DM CSs after 24 h (D) and 4 days (H). No staining was detected in the negative controls (I, J and K).

    Techniques Used: Construct, Cell Culture, Staining, Expressing

    A. In vivo implanted 3 week constructs at harvest (G is acellular GelMA, CSG is biomimetic 3D CSs GelMA construct). B. Bright field images of an in vivo CSG construct. C. Bright field image of an in vivo acellular GelMA constructs.
    Figure Legend Snippet: A. In vivo implanted 3 week constructs at harvest (G is acellular GelMA, CSG is biomimetic 3D CSs GelMA construct). B. Bright field images of an in vivo CSG construct. C. Bright field image of an in vivo acellular GelMA constructs.

    Techniques Used: In Vivo, Construct

    A. No mineralized tissue formation was observed in the acellular GelMA constructs (G). B. Mineralized tissue formation was observed in the CSG constructs. C. 3D model of the mineralized tissue. D. Quantification of mineral density (g/cm3) of the CSG constructs. E. Comparison of mineral densities from engineered and natural mineralized tissues (pig spine, trabecular bone, cortical bone and human enamel) [1, 2]. F. Percent volume of mineralized tissue within ranges of mineral density (ROI – region of interest corresponds to the whole mineralized tissue). G. Representation of areas of mineralized tissue within the ranges of mineral densities (white color represents areas within the range). Abbreviations: MD, mineral density.
    Figure Legend Snippet: A. No mineralized tissue formation was observed in the acellular GelMA constructs (G). B. Mineralized tissue formation was observed in the CSG constructs. C. 3D model of the mineralized tissue. D. Quantification of mineral density (g/cm3) of the CSG constructs. E. Comparison of mineral densities from engineered and natural mineralized tissues (pig spine, trabecular bone, cortical bone and human enamel) [1, 2]. F. Percent volume of mineralized tissue within ranges of mineral density (ROI – region of interest corresponds to the whole mineralized tissue). G. Representation of areas of mineralized tissue within the ranges of mineral densities (white color represents areas within the range). Abbreviations: MD, mineral density.

    Techniques Used: Construct, Comparison

    No tissue formation was observed in the acellular GelMA constructs, H&E (A) and Pol (B) images. H&E stained embedded paraffin and sectioned constructs exhibited high cellularity (C, D), extensive extracellular matrix and dentin/bone-like tissue formation at the DM GelMA layer. The dashed line separates the biomimetic pulp organ (DM in the bottom layer) from the biomimetic enamel organ (DE in the top layer). Pol images (E, F) revealed organized collagen formation within the CSG constructs. IF images (G, H) show the expression of VM (green) by DM cells in the biomimetic pulp organ layer, and ECAD (red) by the DE cells in the biomimetic enamel organ of the CSG constructs.
    Figure Legend Snippet: No tissue formation was observed in the acellular GelMA constructs, H&E (A) and Pol (B) images. H&E stained embedded paraffin and sectioned constructs exhibited high cellularity (C, D), extensive extracellular matrix and dentin/bone-like tissue formation at the DM GelMA layer. The dashed line separates the biomimetic pulp organ (DM in the bottom layer) from the biomimetic enamel organ (DE in the top layer). Pol images (E, F) revealed organized collagen formation within the CSG constructs. IF images (G, H) show the expression of VM (green) by DM cells in the biomimetic pulp organ layer, and ECAD (red) by the DE cells in the biomimetic enamel organ of the CSG constructs.

    Techniques Used: Construct, Staining, Expressing



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    3d gelma biomimetic tooth bud constructs  (BioMimetic Therapeutics)
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    BioMimetic Therapeutics 3d gelma biomimetic tooth bud constructs
    A. DE and DM cells were seeded on thermo-responsive plates and cultured in normal DE and DM media, respectively, for 14 days. DE and DM CSs were detached by temperature reduction (20ºC) and layered over <t>GelMA</t> constructs to create experimental <t>3D</t> tooth bud constructs (CSG = DE and DM CSs layered over dental cells encapsulated in GelMA; G = GelMA alone). For in vivo analyses, replicate constructs were cultured in osteogenic media for 4 days and implanted subcutaneously onto the backs of the rats. B. Bioengineered 3D CS - GelMA tooth bud model. The bottom layer mimics the pulp organ (5% GelMA encapsulating DM cells) and the top layer mimics the enamel organ (3% GelMA encapsulating DE cells). The DE and DM CS layers mimic polarized DE-DM cell layers normally observed in developing teeth. C. Steps used to prepare the constructs. DM cells (3×107 cells/ml) were re-suspended in 100 μL of 5% GelMA and photo-crosslinked. DM and DE cell sheets were layered over the polymerized DM 5% GelMA. DE cells (3×107 cells/ml) re-suspended in 100 μL 3% GelMA and 100 μL, layered over construct and photo-crosslinked.
    3d Gelma Biomimetic Tooth Bud Constructs, supplied by BioMimetic Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3d gelma biomimetic tooth bud constructs/product/BioMimetic Therapeutics
    Average 90 stars, based on 1 article reviews
    3d gelma biomimetic tooth bud constructs - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A. DE and DM cells were seeded on thermo-responsive plates and cultured in normal DE and DM media, respectively, for 14 days. DE and DM CSs were detached by temperature reduction (20ºC) and layered over GelMA constructs to create experimental 3D tooth bud constructs (CSG = DE and DM CSs layered over dental cells encapsulated in GelMA; G = GelMA alone). For in vivo analyses, replicate constructs were cultured in osteogenic media for 4 days and implanted subcutaneously onto the backs of the rats. B. Bioengineered 3D CS - GelMA tooth bud model. The bottom layer mimics the pulp organ (5% GelMA encapsulating DM cells) and the top layer mimics the enamel organ (3% GelMA encapsulating DE cells). The DE and DM CS layers mimic polarized DE-DM cell layers normally observed in developing teeth. C. Steps used to prepare the constructs. DM cells (3×107 cells/ml) were re-suspended in 100 μL of 5% GelMA and photo-crosslinked. DM and DE cell sheets were layered over the polymerized DM 5% GelMA. DE cells (3×107 cells/ml) re-suspended in 100 μL 3% GelMA and 100 μL, layered over construct and photo-crosslinked.

    Journal: Biomaterials

    Article Title: Dental Cell Sheet Biomimetic Tooth Bud Model

    doi: 10.1016/j.biomaterials.2016.08.024

    Figure Lengend Snippet: A. DE and DM cells were seeded on thermo-responsive plates and cultured in normal DE and DM media, respectively, for 14 days. DE and DM CSs were detached by temperature reduction (20ºC) and layered over GelMA constructs to create experimental 3D tooth bud constructs (CSG = DE and DM CSs layered over dental cells encapsulated in GelMA; G = GelMA alone). For in vivo analyses, replicate constructs were cultured in osteogenic media for 4 days and implanted subcutaneously onto the backs of the rats. B. Bioengineered 3D CS - GelMA tooth bud model. The bottom layer mimics the pulp organ (5% GelMA encapsulating DM cells) and the top layer mimics the enamel organ (3% GelMA encapsulating DE cells). The DE and DM CS layers mimic polarized DE-DM cell layers normally observed in developing teeth. C. Steps used to prepare the constructs. DM cells (3×107 cells/ml) were re-suspended in 100 μL of 5% GelMA and photo-crosslinked. DM and DE cell sheets were layered over the polymerized DM 5% GelMA. DE cells (3×107 cells/ml) re-suspended in 100 μL 3% GelMA and 100 μL, layered over construct and photo-crosslinked.

    Article Snippet: In vitro culture and in vivo implantation studies showed that the 3D GelMA biomimetic tooth bud constructs supported DE and DM cell attachment, spreading, metabolic activity, neo-vasculature formation, and mineralized tissue formation of specified size and shape in vivo [ 19 ].

    Techniques: Cell Culture, Construct, In Vivo

    H&E images (A, B, C, D) revealed extracellular matrix formation and the morphology of the DE and DM cell sheets within the bilayer GelMA constructs. The arrows indicate the DE and the DM CSs. Pol images (E, F, G, H) show the organized collagen in the extracellular matrix. IF imaging (I, J, K, L) showed the expression of VM (green) by DM cells and CK18 (red) by the DE cells.

    Journal: Biomaterials

    Article Title: Dental Cell Sheet Biomimetic Tooth Bud Model

    doi: 10.1016/j.biomaterials.2016.08.024

    Figure Lengend Snippet: H&E images (A, B, C, D) revealed extracellular matrix formation and the morphology of the DE and DM cell sheets within the bilayer GelMA constructs. The arrows indicate the DE and the DM CSs. Pol images (E, F, G, H) show the organized collagen in the extracellular matrix. IF imaging (I, J, K, L) showed the expression of VM (green) by DM cells and CK18 (red) by the DE cells.

    Article Snippet: In vitro culture and in vivo implantation studies showed that the 3D GelMA biomimetic tooth bud constructs supported DE and DM cell attachment, spreading, metabolic activity, neo-vasculature formation, and mineralized tissue formation of specified size and shape in vivo [ 19 ].

    Techniques: Construct, Imaging, Expressing

    High magnification H&E images and IHC analyses of multilayered DE DM CSs GelMA constructs cultured in osteogenic media for 24 h and 4 days, stained with FAK, TEN and SYN4. Arrows indicate expression. Cell sheets are identified as epithelial (DE) and mesenchymal (DM). A. H&E stained DE DM CSs GelMA constructs cultured in osteogenic media for 24 h. FAK, TEN and SYN4 staining (B, C and D) were detected in the DM CSs cultured in osteogenic media for 24 h. E. H&E image of DE DM CSs GelMA constructs cultured in osteogenic media for 4 days. F. FAK staining was detected in DE and DM CSs cultured in osteogenic media for 4 days. G. TEN was detected in the DM CSs cultured in osteogenic media for 4 days. H. Faint SYN4 staining was detected in DM CSs cultured in osteogenic media for 4 days. I. No staining was detected in the negative controls. Specific staining was detected on the natural tooth bud (J. FAK, K. TEN and L. SYN4).

    Journal: Biomaterials

    Article Title: Dental Cell Sheet Biomimetic Tooth Bud Model

    doi: 10.1016/j.biomaterials.2016.08.024

    Figure Lengend Snippet: High magnification H&E images and IHC analyses of multilayered DE DM CSs GelMA constructs cultured in osteogenic media for 24 h and 4 days, stained with FAK, TEN and SYN4. Arrows indicate expression. Cell sheets are identified as epithelial (DE) and mesenchymal (DM). A. H&E stained DE DM CSs GelMA constructs cultured in osteogenic media for 24 h. FAK, TEN and SYN4 staining (B, C and D) were detected in the DM CSs cultured in osteogenic media for 24 h. E. H&E image of DE DM CSs GelMA constructs cultured in osteogenic media for 4 days. F. FAK staining was detected in DE and DM CSs cultured in osteogenic media for 4 days. G. TEN was detected in the DM CSs cultured in osteogenic media for 4 days. H. Faint SYN4 staining was detected in DM CSs cultured in osteogenic media for 4 days. I. No staining was detected in the negative controls. Specific staining was detected on the natural tooth bud (J. FAK, K. TEN and L. SYN4).

    Article Snippet: In vitro culture and in vivo implantation studies showed that the 3D GelMA biomimetic tooth bud constructs supported DE and DM cell attachment, spreading, metabolic activity, neo-vasculature formation, and mineralized tissue formation of specified size and shape in vivo [ 19 ].

    Techniques: Construct, Cell Culture, Staining, Expressing

    H&E images and IF analyses of multilayered DE DM CSs GelMA constructs cultured in osteogenic media for 24 h and 4 days, stained with SHH, RUNX2 and BMP2 in green, and VM positive DM cells in red. The red staining identifies the DM CSs, while, the absence of red staining identifies the DE cells. Arrows indicate expression. H&E stained DE DM CSs GelMA constructs cultured in osteogenic media for 24 h (A) and 4 days (E). SHH staining was detected in DE and DM CSs after 24 h (B) and 4 days (F). RUNX2 staining was faintly detected at the interface of DE and DM CSs (C), but strongly detected at the second layer of DE CSs after 24 h (inset in the image C), and detected in DE and DM CSs after 4 days (G). BMP2 staining was detected in DE and DM CSs after 24 h (D) and 4 days (H). No staining was detected in the negative controls (I, J and K).

    Journal: Biomaterials

    Article Title: Dental Cell Sheet Biomimetic Tooth Bud Model

    doi: 10.1016/j.biomaterials.2016.08.024

    Figure Lengend Snippet: H&E images and IF analyses of multilayered DE DM CSs GelMA constructs cultured in osteogenic media for 24 h and 4 days, stained with SHH, RUNX2 and BMP2 in green, and VM positive DM cells in red. The red staining identifies the DM CSs, while, the absence of red staining identifies the DE cells. Arrows indicate expression. H&E stained DE DM CSs GelMA constructs cultured in osteogenic media for 24 h (A) and 4 days (E). SHH staining was detected in DE and DM CSs after 24 h (B) and 4 days (F). RUNX2 staining was faintly detected at the interface of DE and DM CSs (C), but strongly detected at the second layer of DE CSs after 24 h (inset in the image C), and detected in DE and DM CSs after 4 days (G). BMP2 staining was detected in DE and DM CSs after 24 h (D) and 4 days (H). No staining was detected in the negative controls (I, J and K).

    Article Snippet: In vitro culture and in vivo implantation studies showed that the 3D GelMA biomimetic tooth bud constructs supported DE and DM cell attachment, spreading, metabolic activity, neo-vasculature formation, and mineralized tissue formation of specified size and shape in vivo [ 19 ].

    Techniques: Construct, Cell Culture, Staining, Expressing

    A. In vivo implanted 3 week constructs at harvest (G is acellular GelMA, CSG is biomimetic 3D CSs GelMA construct). B. Bright field images of an in vivo CSG construct. C. Bright field image of an in vivo acellular GelMA constructs.

    Journal: Biomaterials

    Article Title: Dental Cell Sheet Biomimetic Tooth Bud Model

    doi: 10.1016/j.biomaterials.2016.08.024

    Figure Lengend Snippet: A. In vivo implanted 3 week constructs at harvest (G is acellular GelMA, CSG is biomimetic 3D CSs GelMA construct). B. Bright field images of an in vivo CSG construct. C. Bright field image of an in vivo acellular GelMA constructs.

    Article Snippet: In vitro culture and in vivo implantation studies showed that the 3D GelMA biomimetic tooth bud constructs supported DE and DM cell attachment, spreading, metabolic activity, neo-vasculature formation, and mineralized tissue formation of specified size and shape in vivo [ 19 ].

    Techniques: In Vivo, Construct

    A. No mineralized tissue formation was observed in the acellular GelMA constructs (G). B. Mineralized tissue formation was observed in the CSG constructs. C. 3D model of the mineralized tissue. D. Quantification of mineral density (g/cm3) of the CSG constructs. E. Comparison of mineral densities from engineered and natural mineralized tissues (pig spine, trabecular bone, cortical bone and human enamel) [1, 2]. F. Percent volume of mineralized tissue within ranges of mineral density (ROI – region of interest corresponds to the whole mineralized tissue). G. Representation of areas of mineralized tissue within the ranges of mineral densities (white color represents areas within the range). Abbreviations: MD, mineral density.

    Journal: Biomaterials

    Article Title: Dental Cell Sheet Biomimetic Tooth Bud Model

    doi: 10.1016/j.biomaterials.2016.08.024

    Figure Lengend Snippet: A. No mineralized tissue formation was observed in the acellular GelMA constructs (G). B. Mineralized tissue formation was observed in the CSG constructs. C. 3D model of the mineralized tissue. D. Quantification of mineral density (g/cm3) of the CSG constructs. E. Comparison of mineral densities from engineered and natural mineralized tissues (pig spine, trabecular bone, cortical bone and human enamel) [1, 2]. F. Percent volume of mineralized tissue within ranges of mineral density (ROI – region of interest corresponds to the whole mineralized tissue). G. Representation of areas of mineralized tissue within the ranges of mineral densities (white color represents areas within the range). Abbreviations: MD, mineral density.

    Article Snippet: In vitro culture and in vivo implantation studies showed that the 3D GelMA biomimetic tooth bud constructs supported DE and DM cell attachment, spreading, metabolic activity, neo-vasculature formation, and mineralized tissue formation of specified size and shape in vivo [ 19 ].

    Techniques: Construct, Comparison

    No tissue formation was observed in the acellular GelMA constructs, H&E (A) and Pol (B) images. H&E stained embedded paraffin and sectioned constructs exhibited high cellularity (C, D), extensive extracellular matrix and dentin/bone-like tissue formation at the DM GelMA layer. The dashed line separates the biomimetic pulp organ (DM in the bottom layer) from the biomimetic enamel organ (DE in the top layer). Pol images (E, F) revealed organized collagen formation within the CSG constructs. IF images (G, H) show the expression of VM (green) by DM cells in the biomimetic pulp organ layer, and ECAD (red) by the DE cells in the biomimetic enamel organ of the CSG constructs.

    Journal: Biomaterials

    Article Title: Dental Cell Sheet Biomimetic Tooth Bud Model

    doi: 10.1016/j.biomaterials.2016.08.024

    Figure Lengend Snippet: No tissue formation was observed in the acellular GelMA constructs, H&E (A) and Pol (B) images. H&E stained embedded paraffin and sectioned constructs exhibited high cellularity (C, D), extensive extracellular matrix and dentin/bone-like tissue formation at the DM GelMA layer. The dashed line separates the biomimetic pulp organ (DM in the bottom layer) from the biomimetic enamel organ (DE in the top layer). Pol images (E, F) revealed organized collagen formation within the CSG constructs. IF images (G, H) show the expression of VM (green) by DM cells in the biomimetic pulp organ layer, and ECAD (red) by the DE cells in the biomimetic enamel organ of the CSG constructs.

    Article Snippet: In vitro culture and in vivo implantation studies showed that the 3D GelMA biomimetic tooth bud constructs supported DE and DM cell attachment, spreading, metabolic activity, neo-vasculature formation, and mineralized tissue formation of specified size and shape in vivo [ 19 ].

    Techniques: Construct, Staining, Expressing